Project 2

Fellow: PhD 2 Host Institution: VUB Start date: October 2014 Position statusCLOSED

Project title: Optimization of freezing protocol (WP1)
Supervisor: E. Goossens, A.M.M. van Pelt (AMC)
PhD enrollment: VUB and UvA
Objectives: The recently established human testicular cell culture system will be characterized to understand, control and optimize the process of SSC propagation in vitro on the molecular, cellular and organ level in respect to various freezing protocols and culture media.
Tasks and Methodology:
a) propagation of human SSCs in vitro;
b) cell characterization;
c) flow cytometry;
d) toxicological testing;
e) next generation sequencing.

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